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Targeting the key questions in the scale development process of TCM syndrome, such as the definition of the concept, the construction of the theoretical framework, the quantitative classification of the items, the rational use of the statistical methods and so on, this article put forward that on the basis of carefully distinguishing the three concepts of syndrome diagnosis, syndrome evaluation and disease diagnosis, and based on TCM dialectical thinking and mathematical validation to build theoretical framework. A scientific and reasonable quantitative classification method was established based on the reliability and validity as indexes. Non-linear intelligent mathematical statistics and symptomatic index groups were used to analyze the ideas and methods of data mining, with a purpose to improve and perfect the methodology of the development of syndromes scale and to improve the establishment and application of the syndrome scale.
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This study was aimed to observe the effect of Yue-Ju Bao-He (YJBH) pill and simvastatin on the reversal of myocardial fibrosis induced by atherosclerosis (AS).ApoE-/-mice were fed on high fat diet to establish models of atherosclerotic plaque and myocardial fibrosis.YJBH pill and simvastatin were used alone or in combination as interventions.Control groups of high fat diet as well as normal diet were observed.Serum lipid and vascular angiotensin Ⅱ (Ang Ⅱ),matrix metalloproteinase-1 (MMP-1) were measured by Elisa.Quantitative analysis of collagen volume fraction (CVF) was conducted by myocardial tissue Masson staining.High-throughput transcriptome sequencing analysis was also adapted to detect differentially expressed genes in myocardial tissues of each group.The results showed that compared with single usage,YJBH pill combined with simvastatin can effectively reduce the total cholesterol (TC),triglyceride (TG),low density lipoprotein (LDL-C) and Ang Ⅱ,increase high density lipoprotein (HDL-C),MMP-1,while Masson staining CVF value was also significantly decreased.Compared with the control group of normal diet,the analysis results from high-throughput transcriptome sequencing analysis showed that difference of gene expression of YJBH pill combined with simvastatin group was mainly in fatty acid metabolism,extend and degradation related pathways,and compared with the model group,the difference of gene expression was mainly in primary immunodeficiency and intestinal immune network IgA production pathways.It was concluded that the combined therapy of YJBH pill and simvastatin can effectively reverse the AS induced myocardial fibrosis.Its mechanism is related to the regulation of immune inflammatory reaction.
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This article analyzed the universality of fatigue existing and the significance of quantization evaluation on fatigue.It expounded the recognition of fatigue in traditional Chinese medicine,and further more proposed the methods of quantization evaluation on fatigue.
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AIM To study the epidermal growth factor (EGF) and transforming growth factor ? (TGF ?)and the epidermal growth factor receptor(EGFR)during the course of rats gastric ulcer natural cure expressing and its possible biology meaning. METHODS We injected acetic acid to make the ulcer animal model, location expression characteristic of EGF and TGF ? and EGFR were studied by immunohistochemistry. RESULTS The normal rats stomach mucosa EGF,TGF ? and EGFR all expressed weakly at masculine. EGF and TGF ? was located at cytoplasm,and EGFR expressesed at cytoplasm and cell membrane and the expression position of the three ovrlopped with each other. EGF concentrated mainly on the cervical part of gastric gland, and TGF ? and EGFR, on the cervical and base parts of the gastric gland. Positive 9 expression cells were primarily parietal[FQ(11?46,X-WZ] and neck cells of the stamook muc. For materials taken during the course of ulcer natural recovery, regular ohanges were observed in TGF, TGF ? and EGFR expression. Higher expression was found in ulcer tissunes than in normal tissues, while the expression was not detectable in necrotic tissues and rarely detectable in granlation and scar tissnes. The expression became apparant only during healing and scar stages of ulcer but not in early stage. CONCLUSION ①TGF ?/EGFR autocrine system probably plays a leading role in maintaining rats mucosa completeness under the normal circumstances; ②EGF/EGFR and TGF ?/EGFR autocrine system may have important effect on the differentiation, hyperplasia, moving of cell and restraining the gastric acidity.